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- 详细信息
- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
- 现货状态:
热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70315125914
中文名称:
英文名称:X1 Heater Block Inserts 3 x 5ml
货期:热销品现货,其他请咨询




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文献和实验Quick Shotgun Cloning of Phage Inserts
(heating block or water bath) heat for 5 min at 65°C (heating block) add 2 ml 5 M NH4 Ac and 10 ml isopropanol spin for 20 min at 4°C wash pellet with 70% EtOH (-20°C), speed vac and dissolve pellet in 4 ml 0.1 x TE (ca 50 ng/ml
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
Anti-GFP/BrdU In Situ Immunostaining
well inserts and cut out mesh insert with a scalpel, forceps and dissecting microscope Place inserts in a 2.0 mL Eppendorf tube with 1 mL of blocking agent (10% goat serum, 1% BSA in PBS). Gently flick tube to mix. Remove solution and wash 3x
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