赛默飞世尔Thermo Fisher METHOD 8260

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

of the partners. View larger version (17K): [in this window] [in a new window]   Figure 1. Summary of the Combined 3C-ChIP-Cloning (6C) method.   For a review of other recent

v468 chapter 5 糖原合成酶激酶-3 的抑制

by the Bradford method. 5. Mix 50 μg of proteins with 30 μL of 2× SDS-gel samplebuffer and then boil for 5 min. 3.2. Sds-page 1. These instructions assume the use of a Hoeffer SE-600 gelsystem. 2. Prepare a 1.5-mm thick, 10% gel by mixing 8.0 mL of 4×

A quick RNA mini-prep for Neurospora mycelial cultures

volume of average-sized sample. Add 0.5 vol. 8 M LiCl, mix and stand at 4°C overnight. 10. Spin at 12000g, 4°C, for 15 min in a fixed angle rotor. Thoroughly resuspend pellet in 4 ml sterile gd (glass distilled) H2 O with pasteur pipette. 11. Extract twice