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英文名称:VISCOMETER,REVERSE FLOW,U- TUBE,SIZE 2,CERTIFIED,
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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
; Fisher BP333) T4 DNA ligase (400 U/µL) TE buffer (pH 8.0) Dilute 10X stock to 1X before use. Triton X-100 (20% v/v; VWR) Trypsin (1X; Invitrogen 25300) Equipment Aluminum foil Cell scraper Centrifuge
Protocols for LCM preparation and analysis
for 15 min Centrifuge 10 min at 40C Transfer upper layer to a new tube Continue with RNA extraction from step 10 Reverse Transcription (final volume 20 uL) Add 24 uL water
Avoiding DNA Contamination in RT-PCR
), a one-step extraction (Tri Reagent), a filter-binding based extraction (RNAqueous™), by centrifugation through a CsCl cushion, and by two rounds of oligo d(T) selection using Ambion's Poly(A)Pure™ Kit (see Figure 1a ). Regardless of whether reverse
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