赛默飞世尔Thermo Fisher POLYGRAM-Fe
rtigfolien IONEX-25 SB-AC Format: 20 x 20 cm (VE=25Stck.) 货号：T_70310489941- ￥1200 - 24000
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310489941
中文名称：
英文名称：POLYGRAM-Fertigfolien IONEX-25 SB-AC Format: 20 x 20 cm (VE=25Stck.)
货期：热销品现货，其他请咨询
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of mannitol over the electrode chamber can not be used for longer that 10 - 15 minutes (it evaporates and the fusion becomes less efficient) and should be changed to fresh one after that time. Pass the group of 20-25 embryos through the drop of mannitol
and mash the fly for 5 - 10 seconds with a pipette tip containing 50 ul of SB, without expelling any liquid (sufficient liquid escapes from the tip). Then expel the remaining SB. Incubate at 25-37 C (or room temp.) for 20-30 minutes
(Engels et al. Cell 62: 515-525). A. DNA PREPARATION PROTOCOL: The squishing buffer (SB) is 10 mM Tris-Cl pH 8.2, 1 mM EDTA, 25 mM NaCl, and 200 ug/ml Proteinase K, with the enzyme diluted fresh from a frozen stock each day.
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