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AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
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- 技术资料
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999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70113-998-011
中文名称:
英文名称:FLAT TABLE MODULE 300 MM
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
thoroughly with warm water and Alconox (Fisher Scientific); cool by filling with liquid N2 . Remove frozen, flat mycelia from foil and add it to the liquid N2 in mortar. Add ~0.5 g of baked sand and grind mycelial pad to a fine powder. Add more N2 as needed
; M. pirum) PCR reagents : Taq polymerase ( 5 U / ul ) dNTP( dGTP, dCTP, dTTP, dATP, 1.25 mM each ) 10x PCR buffer (with 1.5mM MgCl2) 25mM MgCl2 sterile ddH2O Machine / Equipment: PCR thermal cycler agarose电泳设备 DNA电泳胶体观察设备 无菌PCR反应管 无菌1.5 ml
A quick RNA mini-prep for Neurospora mycelial cultures
. 4. Working quickly before powder can thaw, pour or spoon ground mycelia into 15 ml round bottom Sarstedt tube (Sarstedt tubes #60.540, Princeton, NJ) containing 8 ml of E buffer at room temperature. [E buffer: 50 mM Tris-Cl pH 8.0, 300 mM NaCl, 5 mM
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