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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
- 规格:
EA
货号:T_70117-987-95B
中文名称:
英文名称:WIPER SUPRPOL 1500 BULK 150/PK
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文献和实验Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
can be done overnight at RT with constant agitation. 3) Washed stripped membrane at least 3x for 10min each with PBST, then re-block (in PBST+NFDM or PBST+BSA) overnight at 4oC. Stripping Buffer: 62.5mM Tris pH 6.8 2% SDS 100mM b-ME Quick Mix
DTT, 0.5mg/ml BSA 。 18、终止溶液: 95%甲酰胺,20mmol/L EDTA, 0.05%溴酚兰,0.05%二甲苯兰。 19、[a-32P]dATP 或[a-35S]-dATP, 35S 的优点是可使条带为窄而清晰,并且操作更为安全。放射强度为:1000-1500Ci/mmol,10mCi/ml。 20、TE 缓冲液:10mmol/L Tris-HCl,1mmol/L EDTA, pH7.5。 21、上节所述的凝胶制备过程中的全套试剂。 22、X 光显影
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