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文献和实验Large-scale yeast transformation
, 50 mls 10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar from VWR 29442-310, $24/100
storage. PEG: 400 mls 50% PEG, 50 mls 10x TE pH 7.5, 50 mls 1M LiAc pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar
pH7.5. We use relatively fresh mix, but this could be superstition. I guess you could invert, but the capmats don't seal brilliantly. Could use plate sealers (e.g. Corning Costar from VWR 29442-310, $24/100) Agar in omniplates seems to dry
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