赛默飞世尔Thermo Fisher X25 Extract

RNA Extraction from Frozen Tissue Sections

and width of the section. The number of sections needed is then estimated as follows:   Length (mm) ′ width (mm) ′ thickness of sections (mm) ′ # sections = X where X has a value between  and 20 and 100 mm3   For example, a 4′5 mm section

Construction of homemade 'T-vectors'

This method is after Marchuk,D.,et al.,1991,Nucl.Acids Res.19(5),pp1154. You will need:      10 x Taq buffer (Promega)      Taq Polymerase (Promega)      Phenol/chloroform mix      100mM dTTP      TE buffer      Absolute ethanol      70% ethanol

RNA Extraction--RNA提取

will fill 2 ultracentrifuge tubes);2. Add 5 volumes (v/w) homogenization buffer (4M guanidine thiocyanate, 25mM sodium citrate pH 7.0, 1.5% sarcosyl-0.1 M 2- mercaptoethanol added just prior to using) to ground tissue in a beaker or sterile Falcon tube and homogenize