赛默飞世尔Thermo Fisher THYMOL BLUE

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

of 70‐ to 250‐ml volume (e.g., CBS Scientific GM‐150 or GM‐200 or Hoefer SG500) Magnetic stirrers and stir bars Graduated cylinder Adhesive tape 1.5‐mm Teflon combs (10‐, 15‐, or 20‐tooth

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

for the restriction enzyme to be used in Step 16). 13. Pellet the cells by centrifugation. Resuspend in 500 µL of 1.14X restriction enzyme buffer. Transfer to 1.5-mL tubes. 14. Add 7.5 µL of 20% SDS (to a final concentration

v468 Chapter 2 Wnt活性蛋白分离及应用

(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl