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- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70102-617-149
中文名称：
英文名称：PTFE FLAT EVAP DISH 180ML
货期：热销品现货，其他请咨询
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文献和实验In situ hybridisation to alpha satellite sequences (chromosome specific)
flat in a humid chamber (eg a large square plastic petri dish) and drop the pepsin gently onto the slide. Then place in the 37C incubator. This pepsin digestion is the most crucial step. Too little digestion and the probe will not be able to access
Large-scale yeast transformation
transformations (90 mls cells). Grow 50 ml culture in 2xSC-leu 1 , overnight (light innoculum, ie 20 ul of cells). Next morning, add 40 mls YPAD, grow 2-3 hours more. Spin down 100 ul of the culture (=1 transformation's worth) and check
Immunostaining of Paraffin Sections
Procedure: 1) Fix tissues for 3 hr on ice in 4 formaldehyde (2.5 ml of Polysciences #18814 made up to 10 ml in 80 mM NaPO4 [3.2 ml of 1 M NaPO4] pH 6.8 containing 0.2 gm of sucrose). Wash for 2 hr
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