赛默飞世尔Thermo Fisher 样杯 SAMPLE C

常用细胞凋亡检测方法（图）

细胞计 方法： 1、悬浮细胞的染色： （1）收获正常和诱导凋亡的细胞（0.5~1′106），PBS洗2次，1000rpm′10min。 （2）3%多聚甲醛冰浴10min，PBS洗2次，1000rpm′10min。 （3）加入PBS-T溶液，37°C孵育15min，PBS洗2次，1000rpm′10min。 （4）加入200ml胎牛血清，室温反应30min。 （5）加入5ml FITC标记的TFAR19单抗（终浓度为1：40），4℃反应30min （6）荧光细胞洗液洗2次，1000rpm′10

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

, gv3101 - 25GEN 2. Transfer O/N culture to 200ml YEP in a sterile 500ml flask and shake at 250rpm until the OD is 0.3 (4-5hrs) 3. Spin in sterile 50ml screw cap tubes 4C 5krpm 10‚. Check to make sure cells are pelleted, if not repeat at higher

SDS-PAGE

. 0.1% SDS. Keep a squirt bottle of it.VIII. Running buffer: Keep a jug of this. 24 g Tris base, 115.2 g glycine, 20 ml 20% SDS, H2O to 4 liters.IX. Sample buffer: 100 mM Tris, pH 6.8, 2% SDS, 5% ß- mercaptoethanol, 15% glycerol, enough bromophenol blue