赛默飞世尔Thermo Fisher 染色皿 DISH ST

（共享）免疫组织化学染色FAQ

in perfusion fixation ** Michel's fluid for transporting cells or specimens ** Microwave ovens: Advice for new users ** Paraformaldehyde: why won't it dissolve? ** Saccomano's fixative ** Zinc-containing fixatives: What has been published? ** Alternatives

免疫组化石蜡包埋组织抗原修复

for 5 minutes in 100% ethanol Two times for 5 minutes in 95% ethanol Once for 5 minutes in 80% ethanol Approximately, 150 ml of the solutions are enough to cover the removable rack for 10 to 20 slides, using a 105 x 70 x 85 mm coplin jar. Solutions

Immunohistochemisty-Enzymatic Protocol

Bacitracin (Sigma Catalog # B-0125), pH 7.2 PBS: 50 mM Na2HPO4 and 140 mM NaCl, pH 7.2 Incubation Buffer: 1 bovine serum albumin (Sigma Catalog # A2153), 1 normal donkey serum (Sigma Catalog # D9663), 0.3 Triton X-100 (Sigma Catalog # T9284