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英文名称:Crucible porcelain medium form DIN 12904 D 80 mm
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文献和实验): Suspend 10 g of diatomaceous earth (Sigma D-5384) in 100 ml of distilled water in a 100 ml graduated cylinder, and let it settle down for 3 hours. Decant the supernatant, and resuspend the pellet in 100 ml of 6 M guanidine HCl, pH 6.4, containing 50 mM
V294.PART II,Chapter 2 Boyden法体外趋化试验
, 8-μm pore size, 25 × 80 mm(cat. no. K80SH58050; Osmonics; Livermore, CA). 3. MDCK cells (ATCC; cat. no. CCL-34). 4. Dulbecco’s modified Eagle’s medium (DMEM), high glucose, pH 7.4. 5. Fetal bovine serum (FBS). 6. Versene: 0.2 g
1. Sambrook, J., Fritsch, E.F., and Maniatis, T., in Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, NY, Vol. 1, 2, 3 (1989). 2. Glover, D.M. DNA Cloning Volume I: A Practical Approach. IRL Press, Oxford, 1985.
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