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文献和实验in 4% paraformaldehyde to give a working concentration of 10 µg/mL. Discard whatever is not used on the day of use. 6) Grid plates � Purchase from Fisher Scientific (#08-757-149) 7) Na Citrate/Triton X Solution � Dilute Na Citrate in PBS to obtain
Procedures for In Vitro Production of Bovine Embryos
(Fisher)Scalpel blades (#11 and #20) Gloves 400 ml sterile beaker Container to discard ovaries Timer slide warmer @ 38.5°C Dissecting microscope Water-bath at 38.5°CFigure 2. Some culture plates needed for IVP. From left to right are a Falcon 60 x 15
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
from phage- displayed random peptide libraries. J.Biol.Chem. 269(39):23853-23856, 1994. Sambrook, J., Fritsch, E.F., and Maniatis, T. Molecular Cloning. A Laboratory Manual , Cold Spring Harbor, NY:Cold Spring Harbor Laboratory Press, 1989.
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