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货号:T_70312678646
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英文名称:125 ML OCT embedding cryoembedding Matrix 125mL
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文献和实验Staining protocols for placental explant cultures
microscope with fluorescence illumination and long working distance objectives. Whole mount staining in multiwell plates. Method Wash the cultures gently and thoroughly in PBS twice. Fix in 1 ml MeOH. Replace the first solution
Staining protocols for placental explant cultures
objectives. Whole mount staining in multiwell plates. Method Wash the cultures gently and thoroughly in PBS twice. Fix in 1 ml MeOH. Replace the first solution immediately with a second aliquot. Incubate 30 min. Wash three times with PBS. At this stage
Characterization of Corticotropin‐Releasing Factor (CRF) Receptors
5‐ to 15‐ml round‐bottom high‐speed (40,000 × g ) centrifuge tubes (e.g., Fisher) Polytron tissue homogenizer (Brinkmann) or Tissue Tearor (Fisher
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