赛默飞世尔Thermo Fisher X4 BOTTLES
WASH LD POLYETHYLENE WITH INTEGRAL TUBE 250ML (PACK OF 4)(2402-0250) 货号：T_70310464301- ￥70 - 1400
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- 2025年07月12日
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- 详细信息
- 文献和实验
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- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310464301
中文名称：
英文名称：X4 BOTTLES WASH LD POLYETHYLENE WITH INTEGRAL TUBE 250ML (PACK OF 4)(2402-0250)
货期：热销品现货，其他请咨询
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文献和实验(containing 20 ug/ml chloramphenicol for BAC clones and 25 ug/ml kanamycin for PAC clones). This culture has a finite life at 4°C. We recommend that you streak this culture to single colonies on a LB agar plate with an antibiotic and prepare a glycerol stock
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
RNA purification using TRizol--TRIZOL法提取RNA
to a fresh tube. b.Add 0.5 ml of isopropyl alcohol to precipitate RNA. If this RNA will be used for RT-PCR, first add 50 μl isopropyl alcohol, mix, incubate the samples at room temperature for 5 minutes and centrifuge at 12,000 x g for 10 minutes at 4oC
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