赛默飞世尔Thermo Fisher Syringe Fil

Procedures for In Vitro Production of Bovine Embryos

. Prepare 1 M stock by adding 0.735 g CaCl2 +2H2 O to 5 ml water. Sterile filter and store at 4°C indefinitely.Stock 14: Hyaluronidase. Prepare stock solution of type IV hyaluronidase at 10,000 units/ml in saline, sterilize through a 0.2 mm

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of aggregation plate Materials:       Dissecting microscope;       Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3);       1 ml syringe with 26 G 1/2 needle of KSOM medium;       light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);       

Preparation of Embryonic Extract

. 50.2 Ti rotor and tubes, chilled. 14. Millipore filters and holder: type AP, AW, and AA, 47 mm diameter. 15. Cameo 0.22 um filters (Fisher Scientific). 16. 50 ml syringe for applying pressure. 17. 3x 25 ml sterile flasks. 18. 15 ml