Sartorius赛多利斯VS15TR22Vivaspin

Methods for DNA isolation

centrifuge bottle, centrifuge at 10,000 rpm for 30 minutes at 4deg C in the RC5-B using the GSA rotor. For cesium chloride-gradient purification: 7a. Pool the cleared supernatants into to a clean beaker, add one-fourth volume of 50% PEG/0.5 M NaCl

蛋白质印迹（Western blot）实验方案

缓冲液（湿转）25 mM Tris 碱190 mM 甘氨酸20% 甲醇测定 pH，pH 应为约 8.3。必要时进行调节。对于大于 80 kDa 的蛋白质，我们推荐加入最终浓度为 0.1% 的 SDS。转膜缓冲液（半干转）48 mM Tris39 mM 甘氨酸20% 甲醇0.04% SDS封闭缓冲液5% 奶粉或 BSA（牛血清白蛋白）加入 TBST 缓冲液中。混匀并过滤。过滤失败可能导致出现「斑点」，其中微小的黑色颗粒会在显色过程中污染印迹。实验步骤一、样品裂解◇ 制备细胞培养物裂解物1. 将细胞培养皿置于

RNAi和基因沉默的历史回顾

). Stable suppression of gene expression by RNAi in mammalian cells. Proc. Natl. Acad. Sci. USA 99(3):1443-1448. Elbashir SM, Lendeckel W, and Tuschl T. (2001) RNA interference is mediated by 21- and 22-nucleotide RNAs Genes Dev 15(2):188-200. Elbashir SM