sartorius赛多利斯11406--50----ACRC

Methods for the preparation of Fly Extract (FE1 and FE2).

you may have to filter through 0.8µm and 0.45µm filters first. Aliquot to 2.5ml and freeze at -20°C.   Fly Extract 1 for initiating primary culture (FE1) 1. Procedure as for FE2 but the flies

Construction and Characterization of Adenovirus Vectors

stock solution with H2 O and sterilize by autoclaving for 45 min at 121°C. Complete medium Dialysis buffer (10 mM Tris-HCl, pH 8.0) Sterilize by filtration through a 0.2-µm filter. DNase I (Ad) Ethidium bromide

Easy Subcloning

, in which you replace the insert gel slice with 10 µl water. Incubate the ligation overnight at 16℃, or at room temp. for ~3 hours. 10X Ligase buffer 0.5 M Tris pH 7.8 0.1 M MgCl2 50 mM DTT 2 mM EDTA 4 mM rATP Transformation A. Melt the ligation mix in a 70℃