赛默飞Opti-Link系列羧基修饰乳胶微粒，高酸，4.0µ

A safe method of extracting DNA from Coccidioides immitis

in the saprophytic phase at 37 C in 100 ml of 2X GYE medium (20 g glucose and 10 g yeast extract in 1 liter medium, autoclaved for 15 min.) in 500 ml plastic Nalgene flasks with plastic foam stoppers (Identi-plug, Fisher). When there has been sufficient growth

【交流】MICROARRAY入门教材

的替代方法都应先做小规模的试验，并且用于整个点片和杂交过程以确保合适。下面介绍已被成功使用的一种候选方法。 聚丙烯酰胺葡聚糖纯化法：这种方法的原理是用一个简单的凝胶过滤树脂，保留盐离子和其它一些小的分子，而排出PCR产物。因为DNA在水中洗脱而被排出，这样就不需要沉淀过程了。同上所述，操作时必须十分谨慎，以防树脂污染点片溶液。要纯化100µlPCR反应物，准备聚丙烯酰胺葡聚糖S400树脂（Sigma S400 HR）并以2:1的比例用ddH2O稀释。以100µl每等份分装到96孔滤板中

Purification of mAb (IgG)

mlAdd 40 g ammonium sulfate for every 100 ml Sup. (slowly):800gStir O/N in cold room.Pour into plastic bottles. Spin at 4 ℃, 7,500 rpm for 20 min in JA-10 rotor.Prepare 4L 100 mM Tris/pH7.8 (400 ml of 1 M stock/4 L) or Binding buffer.Discard Sup. Resuspend