赛默飞世尔Thermo Fisher YSI 5905-W

Assessment of Substance Abuse Liability in Rodents: Self‐Administration, Drug Discrimination, and Locomotor Sensitization

) Isoflurane Oxygen Saline: 0.9% (w/v) NaCl

Southern Hybridization Protocols

from the hybridization tube into a 50ml centrifuge tube. The probe can be stored frozen for re-use.For room temerperature wash, add in W1. Mix it up and throw W1 solution away in the sink. Repeat twice.Add W2. Leave rotating in the wash at 65℃ for 30 min. Repeat.Add W

Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos

7.0 µl 10x Taq Buffer 2.5 µl 25 mM MgCl2 1.5 µl 10 x dNTP mix 5.0 µl Primer * (30 ng/µl) 5.0 µl Digested DNA (100-200 µg/ml) 2.0 µl mineral oil 40 µl * For cDNAs cloned in Bluscript, we use the following primers (21mers): "T