赛默飞世尔Thermo Fisher VarioPrep H

Convenient and Efficient Approach to the Permanent or Reversible Conjugation of RNA and DNA Sequences with Functional Groups

for 6 hr at 55°C. (C ) Chromatogram of a commercial sample of uridine. Conditions: RP‐HPLC analysis is performed using UV detection (254 nm) and a 5 µm Supelcosil LC‐18S column (25 cm × 4.6 mm) according to the following method: starting from 0.1 M

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

(coarse porosity, Kontes) 4‐mL screw‐cap glass vials (Wheaton) 5‐µm Supelcosil LC‐18S HPLC column (25 cm × 10 mm; Supelco

Time‐Dependent Thermocontrol of the Hydrophilic and Lipophilic Properties of DNA Oligonucleotide Prodrugs

) 5‐µm Supelcosil LC‐18S HPLC column (25 cm × 4.6 mm, Supelco) 37°C water bath 90°C Heat block (VWR