赛默飞世尔Thermo Fisher Blotting me
mbrane Whatman Nytran N roll 0.45µm pore sizemoderately charged Nylon 300mmx 3m 货号：T_70310003401- ￥1200 - 24000
- Thermo Fisher
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- T_70310003401
- 2025年07月14日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70310003401
中文名称：
英文名称：Blotting membrane Whatman Nytran N roll 0.45µm pore sizemoderately charged Nylon 300mmx 3m
货期：热销品现货，其他请咨询
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文献和实验Semi-dry Transfer Apparatus Biorad, Cat# 170-3940, or equivalent Power Supply 0 - 100 VDC (adj. current to 1 Amp) Immobilon-Ptransfer membrane 0.45 µm pore size; cut to same size as gel (Millipore, Cat# IPVH304-FO, or equivalent) Filter Paper
Southern Blot Analysis(from Baker lab, university of Florida)
minutes.Denatures DNA for probe access.3.Prepare 2 liters of 0.025M NaPO4 pH6.5 transfer buffer (25ml 0.5M Na2HPO4,75ml 0.5M NaH2 PO4 / 2 liter).4.Cut Hybond N to the size of the gel and wet with transfer buffer (Gloves a must).5.Cut 4 pieces of Whatman
Southern Blot Analysis(from Baker lab, university of Florida
gloves roll out bubbles. 10.Cut four striPS of saran wrap and place over the edges of the gel. This is to prevent the buffer from "short -circuiting". So that the buffer flows through rather than around the gel. 11.Place Hybond N+ membrane exactly
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