赛默飞世尔Thermo Fisher PRE-TREATME

Pre-PCR Processing of Samples

. However, the usefulness of diagnostic PCR is limited, in part, by the presence of inhibitory substances in complex biological samples, which reduce or even block the amplification capacity of PCR in comparison with pure solutions of nucleic acids ( 1

Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos

as stage X, nearly 24 h earlier in development than most electroporation protocols. Compared to in ovo electroporation, the ex ovo method allows easier embryo orientation (the posterior half of the embryo is darker than the anterior half

一篇搞定 IP、co-IP 实验

的，但是这结果是不是假阳性呢？还得做个阴性对照。阴性对照如何设置呢？考虑到整个实验体系会出现 beads、抗X抗体、诱饵蛋白 X、靶蛋白 Y（co-IP）： 可能出现的假阳性来源 需要的阴性对照 抗 X 抗体结合非特异的蛋白 与 IP 一抗种属亚型相同的免疫球蛋白（比如，抗 X 抗体是 Mouse IgG，阴性对照可使用 Mouse 免疫球蛋白） beads 对蛋白的非特异结合* 同上，此外还可进行 Pre clear，IP 正式实验前排除非特异结合蛋白。如果使用琼脂