赛默飞世尔Thermo Fisher X25 Reservo

小RNA的Northern BLOT

#______________  1 μl T4 Polynucleotide Kinase, 10 U/μl Lot#______________  X μl Nuclease free H2O to 10 μl  37℃ at least 1 hr  After incubation, bring reaction volume up to 50 μl before cleaning up with spin column

On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

in strong dispersion of the ITP interface.14     1)      To decontaminate the channel, fill the North, East, and South reservoirs with 10-20 μL of 10% bleach and apply vacuum at the West reservoir for 2 min. If using a standard Caliper chip caddy

miRNA microarrays（McManus Lab）(芯片）

Ambion 3x hybridization buffer (Cat # 1567 mirVana™ miRNA Bioarray Essentials Kit) 22x25 mm lifter sliPS (Erie 22x25I-M-5226) a) Heat 3x hyb buffer at 65℃ and vortex to resuspend b) Add 5 to 10 100 μL drops of 5x SSC