赛默飞世尔Thermo Fisher X250 Filter

Procedures for In Vitro Production of Bovine Embryos

for one week only.Stock 20: PO 4 . Dissolve 0.235 g NaH2 PO4 +H2 O in 50 mL water. Sterile filter and store at 4°C.Stock 21: 1 M HEPES. Add 119 g of HEPES to 400 mL water. Adjust pH to 7.0 and bring volume up to 500 mL. Sterile

Genomic DNA Extraction - PureLink

cell lysate Zymolase buffer Zymolase (lyticase) enzyme for yeast lysate Sterile, DNase-free microcentrifuge tubes Water baths or heat blocks Components supplied with the kit Lysis Buffer (L6) Binding Buffer (L3

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

. DNA preparations DNA for electroporation must be free of salt, RNA or protein. DNA (in TE buffer) should be first treated with RNase, then twice extracted with phenol/chloroform. This will remove protein and RNA. To remove salt, EtOH