赛默飞世尔Thermo Fisher X96 Collect

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

with 0.5x MBS at RT. (5)   Transfer tissues into a 1.5 ml microfuge tube using a wide-bore plastic pipette. Remove the MBS as much as possible. (6)  You may store the tissues at �80°C (use liquid nitrogen to freeze them). (7)  Add 0.5 ml of �

Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions

) 10‐ml Sephadex G25 gel filtration column (essentially any standard desalting column can be used) Amicon Ultra centrifugal concentrators (MWCO, 10,000; Millipore, cat

Methods for DNA isolation

or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture