60209-003赛默飞SOLAµ2mg/1mL 96 孔板

Adaptation of Stem Cells to 96‐Well Plate Assays: Use of Human Embryonic and Mouse Neural Stem Cells in the MTT Assay

cells (hESC) are difficult to adapt to 96?well plate assays, such as the MTT assay, because they survive best when plated as colonies, which are not easily counted and plated accurately. Two methods were developed to address this problem

双荧光素酶实验怎么做？实验设计、共转染与结果归一化

1. 96孔板可从2.5×10⁴ cells/well建立条件 细胞数量需要与所用细胞系和转染试剂匹配。以HEK293细胞配合Lipofectamine LTX为例，96孔板参考条件为2.5×10⁴ cells/well、培养液100 μL/well，转染当天细胞融合度保持在50%～80%；对应总DNA量为100 ng/well，Lipofectamine LTX为0.15～0.35 μL/well。 这一组参数可以作为96孔体系建立时的起始参考。更换细胞系或转染试剂后，细胞数量、DNA量和转染

细胞吸附试验

一、材料和仪器 1. 海拉细胞（ATCC#CCL-2）2. 96孔聚苯乙烯板（Fisher#07-200-91;Corning#3598）3. MTT细胞增殖试剂盒（ATCC#30-1010K）4. 胶原I（Sigma-Aldrich#C7661）5. 密理博（D-MEM）高糖培养基DMEM（Invitrogen#10313-021）6. 胎牛血清（ATCC#30-2020）7. 0.5 M EDTAsolution8. 牛血清蛋白（Invitrogen#15561