- ￥658 - 2280
- Tanda
- 中国
- TD-200921
- 2025年12月05日
- WB,IP
- Mouse
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
peptide
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
-20℃
- 克隆性：
mAb
- 适应物种：
Human
- 保质期：
1年
- 目录编号：
TD-200921
- 级别：
科研级
- 库存：
88
- 供应商：
武汉天德
- 宿主：
Mouse
- 应用范围：
WB,IP
- 靶点：
详询
- 抗体英文名：
CD31 (2D3) Mouse mAb
- 抗体名：
CD31
- 规格：
100ul/50ul/20ul
| 规格： | 100ul | 产品价格： | ￥2280.0 |
|---|---|---|---|
| 规格： | 50ul | 产品价格： | ￥1280.0 |
| 规格： | 20ul | 产品价格： | ￥658.0 |
CD31 (2D3) Mouse mAb
|
Product Name
|
CD31 (2D3) Mouse mAb
|
|
Gene ID
|
5175
|
|
SwissProt ID
|
P16284
|
|
Gene Name
|
PECAM1
|
|
Alternative Names
|
Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31
|
|
Background
|
PECAM-1 a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues.
|
|
Research Field
|
Cardiovascular
|
|
Product Categories
|
Primary antibody
|
|
Host
|
Mouse
|
|
Reactivity
|
Human
|
|
Application
|
WB,IP
|
|
Dilution Ratio
|
WB: 1/500-1/1000 IP: 1/20
|
|
Molecular Weight
|
Calculated MW: 83 kDa; Obs/erved MW: 130 kDa
|
|
Clonality
|
Monoclonal Antibody
|
|
Clonality No.
|
2D3-B4-C8
|
|
Isotype
|
IgG1
|
|
Immunogen
|
Purified recombinant human CD31 protein fragments expressed in E.coli.
|
|
Purification
|
Affinity Purified
|
|
Conjugation
|
Unconjugated
|
|
Modification
|
Unmodified
|
|
Form
|
Liquid
|
|
Buffer System
|
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.
|
|
Storage
|
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
|
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
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