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- 文献和实验
- 技术资料
- 保存条件:
Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month.
- 英文名:
2-Hydroxyquinoline
- 库存:
国内:现货
- 供应商:
MedChemExpress LLC
- CAS号:
59-31-4
- 规格:
1 g/5 g/10 g
| 规格: | 1 g | 产品价格: | ¥100.0 |
|---|---|---|---|
| 规格: | 5 g | 产品价格: | ¥196.0 |
| 规格: | 10 g | 产品价格: | ¥288.0 |
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文献和实验Deproteination using phenol/chloroform
'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydroxyquinoline added to a final concentration of 0.1%, and equilibrated three times with an equal volume of 1M Tris.HCl, pH 7.0. The final Tris wash is replaced with TE (10
Deproteination using phenol/chloroform
'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydroxyquinoline added
DNA Purification and Precipitation
1.Prepare or obtain Buffered phenol,pH 8.0.Add 2 crystals of 8-Hydroxyquinoline to prevent oxidation.This also identifies the organic phase as yellow-colored. 2.Combine DNA sample with an equal volume of Buffered Phenol. 3.Mix well by vortexing
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