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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
BSA PE Polypropylene Particles, 15 um
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验Western Blotting with Horseradish Pe
) is now ready for loading onto your gel. For Protein Concentration Determination of Whole Tissue 1.Rapidly homogenize every 0.25g tissue in 3.5ml of boiling lysis buffer (1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris pH 7.4). 2.Microwave for 10–15 seconds
T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
PriCells: 藻红蛋白标记抗体的方法﹠PE-标记蛋白A方法
藻红蛋白标记抗体的方法:1. 巯基化藻红蛋白(PE)的制备;(1)将600μl浓度为15.5mg/ml的盐酸巯醇亚胺加到1.2ml浓度为3.6mg/ml的PE中;(2)将以上混合液和1.2ml pH6.8的PB混合,而后装入透析袋置入50mmol/L pH6.8的PB中于4℃透析,过夜;(3)再换用pH7.5 PB透析6h。每个PE分子中可结合8个巯基;2. 巯基PE-IgG的制备;(1)将30μl浓度为1.1mg/ml的异双功能试剂SPDP的乙醇溶液,加入到700μl
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