- ￥2500
- 百欧泰(biotyscience)
- 北京
- ABT-31-0002-Protein G
- 2026年04月12日
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- 文献和实验
- 技术资料
- 英文名：
Protein G PMMA Particles
- 库存：
100
- 供应商：
百欧泰(biotyscience)
- 规格：
10 mg/ml10ml
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
of various immunoglobulines. Protein A is a 42 kDa polypeptide that binds the residues in the CH2 and CH3 domains of immunoglobulin heavy chains. Protein G is an approximatley 30 kDa polypeptide that binds immunoglobulins and albumin. Recombinant protein G
Coupling Antibodies to Protein A or G
1. use 2 mg of antibody per ml wet beads (use appropriate antibody/protein A or G combination). 2. mix antibodies with beads and bind at room temperature for at least 1 hr (on roller). 3. wash the beads twice with 10 volumes borate buffer, spin
Protein G Purification of Antibodies
1. Reagent and Materials(1) Hi-Trap Protein G Column (Pharmacia Biotech #17-0404-01)(2) 20 mM Sodium Phosphate Buffer, pH 7.01.084 g NaH2PO4, anhydrous3.273 g Na2HPO4.7H2Oq.s. to 1 liter with di-H2O(3) 0.1M Glycine, pH 2.83.75 g Glycine1.4 ml HCl
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