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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Protein A PMMA Particles
- 库存:
100
- 供应商:
百欧泰(biotyscience)
- 规格:
10 mg/ml10ml
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文献和实验UV Absorbance (280 nm) Protein Determination
UV Absorbance (280 nm) � Protein Determination Simple and quick method to accurately quantitate total protein in purified material or approximately quantitate total protein in crude lysates or partial purified material
Procedure to purify protein from cells
This procedure is used to break cells that contain a protein to be purified.This procedure is valid only for the use on Appling's lab sonicatior. PREPARATION 1.Place your sample in a 15ml elongated plastic tube such that the sample-tube wall
triton X (1% final concentration). Place tubes in rotary shaker at 4°C for 15 minutes. Spin cells for 15 minutes at 7000 RPM, collect supernatant into Beckman centrifuge tube. Spin again for 30 minutes at 45 K. Separate supernatant (our protein is soluble
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