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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存
- 英文名:
Trypsin from bovine pancreas(2X,Sterile,Irradiated)
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
T128772-100mg
Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins
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文献和实验酸氨基转移酶(alt)、总蛋白(tp)、白蛋白(alb)、总胆红素(tb)、尿素(un)、肌酐(cr)、血糖(glu)、血镁(mg)、肌酸激酶(ck)、肌酸激酶同功酶(ckmb)10个项目进行检测,并进行了结果比对和相关回归分析,确定预期偏差是否在允许的范围内。 1 材料与方法 1.1 仪器与试剂 美国强生公司生产的vitros 350干化学分析仪(简称强生v-350)及配套试剂、标准品;日立7600生化仪,alt、tb、cr、mg试剂及配套校准
(1 mg will inhibit 1 mg of trypsin with an activity of 10,000 BAEE units/mg protein). T6552 is of higher purity (1 mg will inhibit 1.0-3.0 mg ot trypsin with an activity of 10,000 BAEE units/mg protein), but more importantly, it has been suitability
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
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