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- 询价记录
- 文献和实验
- 技术资料
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 英文名:
Recombinant Human FGF basic/FGF2/bFGF Protein
- 规格:
rp155946-1mg/rp155946-50μg/rp155946-100μg/rp155946-10μg
| 规格: | rp155946-1mg | 产品价格: | ¥6399.90 |
|---|---|---|---|
| 规格: | rp155946-50μg | 产品价格: | ¥569.90 |
| 规格: | rp155946-100μg | 产品价格: | ¥909.90 |
| 规格: | rp155946-10μg | 产品价格: | ¥289.90 |
基本描述
产品名称:Recombinant Human FGF basic/FGF2/bFGF Protein
规格或纯度:无动物源,无载体,生物活性,ActiBioPure™,高性能,≥95%(SDS-PAGE)
应用:SDS-PAGE, Cell Culture, Functional Studies
产品介绍:
碱性成纤维细胞生长因子(bFGF,FGF2或FGF-β)是成纤维细胞生长因子家族中的一员。bFGF是一种单链多肽生长因子,在促进细胞增殖过程中起着重要作用;同时也可以抑制细胞凋亡,加速伤口愈合并诱导血管再生。在中胚层和神经外胚层等多种类型的细胞中,bFGF亦能非常有效地诱导DNA的合成。OsrhbFGF是运用基因重组技术,由水稻胚乳细胞表达并通过蛋白提纯工艺而成。
生物活性:Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
纯度:≥95% (SDS-PAGE)
氨基酸:134-288 aa
蛋白长度:Full length protein
无载体:Yes
Accession #:
预测分子量:17 kDa
级别:无动物源, 无载体, 生物活性, ActiBioPure™, 高性能
产品规格参数
储存缓冲液:Human basic fibroblast growth factor, L-glutathione, Disodium edesylate, Sodium dihydrogen monohydrate phosphate, Disodium hydrogen dodecahydrate phosphate, Sodium chloride, Mannitol, H2O
复溶:It is recommended to redissolve the lyophilized powder in sterile water to 100-200 μg/ml, and further dilute it in other solvents.
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:Store at -20 ℃ for 36 months. Upon delivery aliquot. Avoid freeze/thaw cycle. Upon reconstitution, the product should be stored at 2-8 ℃ for 2-7 days and used up as soon as possible. For future use, should be stored at -20 ℃.
CAS编号和信息:62031-54-3
功能和特点
Accession #:
生物活性:Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically <1 ng/mL.
图片
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Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp155946) - Protein Bioactivity
Measured in a cell proliferation assay using BALB/3T3 mouse embryo fibroblasts cell line. The ED₅₀ for this effect is typically less than 1.0 ng/mL.
>
Recombinant Human FGF basic/FGF2/bFGF(155aa) Protein (rp155946)-SDS-PAGE
3μg/lane of Recombinant Human FGF2 was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 16.0 kDa.
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- 作者
- 内容
- 询问日期
文献和实验Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
at 200 x g for 4 minutes. Remove the supernatant and gently flick the pellet. Resuspend the pellet in an appropriate amount of Human Feeder Cell Conditioned Medium supplemented with 4 ng/mL of recombinant human FGF basic. Add
Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
(approximately 1.0 x 106 cells/60 mm plate) on the Cultrex BME coated plate in Conditioned Media containing 4 ng/mL of recombinant human FGF basic. Change the media daily. Monitor the cells for the desired confluency. Figure
by a cell proliferation assay using human AML5 cells is less than 2.0 ng/mL. 相关产品信息 产品名称 货号 规格 Recombinant Human Flt-3 Ligand GMP Protein GMP级重组人FLT3配体 92358ES 10μg/50μg/1mg Recombinant Human Flt-3 Ligand Protein 重组
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