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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存,避免反复冻融;特低温运输
- 英文名:
C1q Protein in 40 % Glycerol
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
C409521-1000μg/C409521-250μg/C409521-50μg
| 规格: | C409521-1000μg | 产品价格: | ¥8799.90 |
|---|---|---|---|
| 规格: | C409521-250μg | 产品价格: | ¥3499.90 |
| 规格: | C409521-50μg | 产品价格: | ¥1199.90 |
基本描述
产品名称:C1q Protein in 40 % Glycerol
规格或纯度:1.0 mg/mL,0.22 µm filtered
生物活性:> 1,000,000 C1H50 units/mg
产品规格参数
储存缓冲液:40% glycerol, 10 mM HEPES, 300 mM NaCl, pH 7.2
浓度:1.0 mg/mL,0.22 µm filtered
储存条件:-80℃储存,避免反复冻融
运输条件:特低温运输
功能和特点
生物活性:> 1,000,000 C1H50 units/mg
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. Shake rack thoroughly during the washing procedure. iv. Rinse slides in PBS. Add 100 µL of a solution containing 0.5 mg/mL DAB and 0.01% H2 O2 . v. To prevent overstaining, follow the appearance
Bacterial Protein Extraction (mini-scale)
buffer (25mM TrisHCl pH8.0; 0.1M NaCl; 10% glycerol; 0.1% Triton X-100 and 1mM PMSF).Spin 13000rpm 5min 4℃,to separate detergent-soluble proteins from inclusion bodies in the pellet.Collect supernatant.Keep 40μl sample of supernatant for PAGE-SDS
Protein Expression and Purification of RPA70-AB (181-422)
of protein1.Pick a single colony [freshly transformed Rosetta BL21 (DE3)cells,(available from Novagene)or from a glycerol stock] in 2 ml LB containing 20 µg/ml Kanamycin (Kan)and 34 µg/ml of chloramphenicol (Cam)and grow cells until OD600 (0.4~0.6)at 37 ℃.2
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