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- 详细信息
- 技术资料
- 免疫原:
A synthetic peptide derived from human DAB2 (AA 251-370)
- 亚型:
Rabbit IgG
- 保存条件:
-20°C储存,避免反复冻融;超低温运输
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 浓度:
0.7 mg/mL
- 规格:
Ab099105-100μl/Ab099105-10μl/Ab099105-1ml/Ab099105-50μl
| 规格: | Ab099105-100μl | 产品价格: | ¥2099.90 |
|---|---|---|---|
| 规格: | Ab099105-10μl | 产品价格: | ¥499.90 |
| 规格: | Ab099105-1ml | 产品价格: | ¥16999.90 |
| 规格: | Ab099105-50μl | 产品价格: | ¥1299.90 |
基本信息
产品名称:Recombinant DAB2 Antibody
规格或纯度:ExactAb™, Validated, 重组, 0.7 mg/mL
应用:WB, IF/ICC, IHC
特异性:DAB2
种属反应性:人(Human), 大鼠(Rat)
免疫原:A synthetic peptide derived from human DAB2 (AA 251-370)
阳性对照:WB: HeLa, U2OS, HUVEC and C6 cell lysates. IHC: Human lung cancer tissue. IF/ICC: HeLa cells.
偶联:Unconjugated
产品属性
克隆类型:重组抗体
Format:Whole IgG
亚型:Rabbit IgG
SDS-PAGE:150 kDa
纯化方法:Antigen affinity purified
物理外观:Liquid
储存缓冲液:50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂:0.01% Sodium azide
浓度:0.7 mg/mL
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 大鼠(Rat)
偶联:Unconjugated
图片
Recombinant DAB2 Antibody (Ab099105) - Western Blot
All lanes: Recombinant DAB2 Antibody (Ab099105) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 82 kDa
Observed band size: 99 kDa
Exposure time: 110.1 s
Recombinant DAB2 Antibody (Ab099105) - Western Blot
All lanes: Recombinant DAB2 Antibody (Ab099105) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 82 kDa
Observed band size: 99 kDa
Exposure time: 10.0 s
Recombinant DAB2 Antibody (Ab099105) - IHC
Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Recombinant DAB2 Antibody (Ab099105). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Recombinant DAB2 Antibody (Ab099105) - IF/ICC
IF analysis of Recombinant DAB2 Antibody (Ab099105) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant DAB2 Antibody (Ab099105) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF647) (Ab181958) at a dilution of 1/1000 for 1 hour at room temperature in the dark (red). Cells were counterstained with DAPI (blue).
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