- ￥3060.90
- 阿拉丁
- 上海
- S598153
- 2026年01月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
避光;-20°C储存
- 英文名：
Sulfo-cy3-3 free acid
- 库存：
期货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
S598153-5mg
英文名：Sulfo-cy3-3 free acid
纯度或规格：0
SPU：S598153
CAS：-
存储温度：避光;-20°C储存
运输条件：超低温运输
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文献和实验Dual- and Triple-Color Fluorospot
for the other cytokine (Fig. 1). As a second step in the detection, streptavidin conjugated to a red fluorophore (Cy3) and anti-FITC antibodies labeled with a green fluorophore, respectively, are used (10, 11). The resulting spots are subsequently analyzed using
were prepared by labeling BSA with Cy3 and Cy5 monofunctional reactive dyes (Amersham Pharmacia Biotech). Cy5-BSA-biotin was prepared by labeling Cy5-BSA with Sulfo-NHS-LC-Biotin (Pierce). Cy3-BSA-maleimide was prepared by labeling Cy3-BSA with Sulfo-GMBS
Amino-allyl Reverse Transcription
Resuspend the contents of one pack of the monofunctional NHS-ester Cy3 or Cy5 dye (mono-functional Cy3 or Cy5 reactive pack, Amersham catalog PA 23001 and PA 25001) in 72 ul DMSO. Aliquot 16 x 4.5 ul and either use immediately or dry
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