- ￥5688.90
- 阿拉丁
- 上海
- S598307
- 2026年01月06日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
避光;-20°C储存
- 英文名：
Sulfo-cy3-e-dutp (sulfo-cy3-ethyl-dutp)
- 库存：
期货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 规格：
S598307-25nmol
英文名：Sulfo-cy3-e-dutp (sulfo-cy3-ethyl-dutp)
纯度或规格：0
SPU：S598307
CAS：-
存储温度：避光;-20°C储存
运输条件：超低温运输
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文献和实验Revised amino-allyl labeling protocol
at RT in the dark. Mix every 15 minutes. E. Quenching and Cleanup Before combining Cy3 and Cy5 samples for hybridization, unreactive NHS-ester Cy dye must be quenched to prevent cross coupling. Add 4.5 m L 4M hydroxylamine (Sigma
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
the chemical reaction. Aliquot unused dye and dry immediately. Incubate 1 hour at RT in the dark. Mix every 15 minutes. E. Quenching and Cleanup Before combining Cy3 and Cy5 samples for hybridization, unreactive NHS-ester Cy
concentration klenow work as well. 1. Add 2 ug DNA of the sample to be labeled to an eppindorf tube. Note: For high complexity DNAs (e.g. human genomic DNA), the labeling reaction works more efficiently if the fragment size of the DNA
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