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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存;充氩
- 英文名:
NHS-PEG-NHS
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
N164060-500mg/N164060-100mg
| 规格: | N164060-500mg | 产品价格: | ¥981.9 |
|---|---|---|---|
| 规格: | N164060-100mg | 产品价格: | ¥276.9 |
N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.
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文献和实验CD35 分子 CD35 常用单克隆抗体或代号: TO5,E11;(CR1) 主要表达细胞: G,M,DC,B,NKsubRBC [M] 分子质量(kDa)和结构: p250(含CCP结构域) 功 能: 结合C3b和C4b,调理吞噬,红细胞免疫粘附,调节B细胞活化 CD35 Aka CR1, C3b and C4b receptor Mediates adherence
Labeling Gizzard Myosin with Tetramethylrhodamine Iodoacetamide (Primarily 17kDa Light Chain)
. 5.Resuspend pellets in 3-5 ml of buffer 2,centrifuge as in step 4. 6.Resuspend pellets in a small volume of buffer 3. 7.Dialyze overnight against buffer 4. 8.Centrifuge in a 50Ti rotor at 35,000 rpm,4℃,for 60 min.The supernatant is good for microinjection
网络 二、I型补体受体(CD35) I型补体受体(CR1、C3b/C受体,又称CD35) 为单链穿膜糖蛋白,分子量160-260kDa。CRI有四种同种异型,其分子量与基因频率分别为,A:190kDa(0.83)、B:220kDa(0.16)、C:160kDa(0.01)和D:260kDa(0.002),但它们的功能相同。CR1广泛分布
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