Cyanine3 NHS酯，2632339-91-2，≥95

Cyanine Dye Purification Protocol

Slowly add 111.3 mL (80.95 g) HPLC grade triethylamine; the reaction is exothermic; the solution will become cloudy � wait until it clears before adding more) Add dropwise an additional 10-15 mL acetic acid to adjust the pH to 8

Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes

of substituted unsymmetrical cyanine SYTO probes has recently become commercially available. Derived from thiazole orange, SYTO display low intrinsic fluorescence, with strong enhancement upon binding to DNA and/or RNA. Broad selection of excitation/emission

流式课堂 | 流式实验对照设置之 FMO

大部分老师做流式实验时，会用到空白对照、单阳对照、同型对照、生物学对照等，用到 FMO 对照的比较少。尽管如此，FMO 对照在设置圈门时仍具有重要的参考价值。 一方面，FMO 对照有助于精确地设置阴阳性群体界限。我们在做多色实验时，可能会出现某些通道信号难以区分的情况，主要是因为其他某个荧光对该通道的干扰很大。FMO 对照（Fluorescence Minus One，荧光扣除一）减少了其中一个通道的荧光抗体，能够帮助衡量其它通道的荧光漏溢情况。 如下图所示，PerCP/Cyanine