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Chikungunya
Relating to the geographic occurrence the virus is divided into five variations: the West-African, the East- and South-African, the one of the Indian Ocean and the Asiatic.
Chikungunya-Viruses are transmitted to humans by bloodsucking mosquitoes
(Aedes aegypti, Aedes albopictus). In general the viruses are not transmitted from human to human but transmissions from infected pregnant women to unborn children have been proved.
The symptoms of Chikungunya include fever which can reach 39°C (102,2°F) a petechial or maculopapular rash usually involving the limbs and trunk, and arthralgia or arthritis affecting multiple joints which can be debilitating.
The symptoms can also include headache, conjunctival injection and slight photophobia. The fever typically lasts for two days and abruptly comes down. However other symptoms, namely joint pain, intense headache, insomnia and an extreme degree of prostration last for a variable period, usually for about five to seven days. But patients have complained joint pain for much longer time period depending on age of the patient. It has been observed that the severity of the disease as well as its duration is less in younger patients and pregnant women. Heavy damages to somebody’s health or death are rare.
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文献和实验Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
the instructions provided with the kit. Note: Because the CytoTune™ Sendai reprogramming vectors are based on SeV, which is an RNA virus, reverse transcription is required for detecting the presence of the SeV genome in your reprogrammed cells. c
A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
测定,可以采用竞争法模式。其原理是标本中的抗原和一定量的酶标抗原竞争与固相抗体结合。标本中抗原量含量愈多,结合在固相上的酶标抗原愈少,最后的显色也愈浅。小分子激素、药物等ELISA测定多用此法。2.2.6 捕获包被法测抗体(经典方法)IgM抗体的检测用于传染病的早期诊断中。间接法ELISA一般仅适用于检测总抗体或IgG抗体。如用抗原包被的间接法直接测定IgM抗体,因标本中一般同时存在较高浓度的IgG抗体,后者将竞争结合固相抗原而使一部份IgM抗体不能结合到固相上。因此如用抗人IgM作为二抗,间接测定IgM
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