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LIGHT DIAGNOSTICS? Respiratory Virus Panel I DFA, ~50 tests
Description:
LIGHT DIAGNOSTICS? Respiratory Virus Panel I DFA, ~50 tests
Trade Name:
LIGHT DIAGNOSTICSChemicon (Millipore)
Product Overview:
The Light Diagnostics Respiratory Panel 1 DFA is intended for the qualitative detection and identification of adenovirus, influenza A, influenza B, respiratory syncytial virus (RSV), parainfluenza 1, parainfluenza 2 and parainfluenza 3 following amplification in cell culture.
For In Vitro Diagnostic Use
Test Principle:
Light Diagnostics Respiratory Panel 1 DFA utilizes direct immunofluorescence to identify virus in patient specimens and/or cell cultures. The fluorescein-labeled monoclonal antibody will bind to the appropriate viral antigen on the slide, if present. Unbound monoclonal antibody is removed by washing with a phosphate-buffered saline (PBS)/Tween Solution. The antigen-antibody complex will show apple-green fluorescence by fluorescence microscopy. Uninfected cells stain a dull red due to the presence of Evans Blue in the reagents.
Light Diagnostics Respiratory Panel I Kit utilizes monoclonal antibodies for the detection and identification of RSV, influenza A, influenza B, adenovirus, parainfluenza 1, 2 and 3 from patient samples following amplification in culture.
Key Applications:
Immunofluorescence
Usage Statement:
For in vitro Diagnostic Use
CE Mark
Kit or Assay Type:
Diagnostics Kits
Components:
DFA Reagents - (Catalog No. Adeno - 5016, Influenza A - 5017, Influenza B - 5018, Para 1 - 5019, Para 2 - 5020, Para 3 - 5021, RSV - 5022). Seven (7) x 2 mL dropper vials containing fluorescein-labeled monoclonal antibody directed against: adenovirus, influenza A, influenza B, parainfluenza 1, parainfluenza 2, parainfluenza 3, and RSV with protein stabilizer, Evans blue and 0.1% sodium azide (preservative).
Antigen Control Slides - (Catalog No. Adeno - 5009, Influenza A & B - 5010, Para 1,2,3 - 5011, RSV - 5012). Four (4) control slides containing wells with positive and negative control cells. Each positive control well is infected with one of the following: adenovirus, influenza A, influenza B, parainfluenza 1, parainfluenza 2, parainfluenza 3, and RSV. Negative control wells contain uninfected cells.
Phosphate-Buffered Saline - (Catalog No. 5087). One (1) packet of PBS salts.
Tween 20 / Sodium Azide Solution (100X) - (Catalog No. 5037). One (1) 10 mL vial containing Tween 20 / Sodium Azide concentrate.
Mounting Fluid - (Catalog No. 5013). One (1) 10 mL dropper vial containing Tris-buffered glycerin, a fluorescence enhancer and 0.1% sodium azide (preservative).
Format:
FITC
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