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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human EpCAM Protein (ECD, His Tag, Site-Specific AF488 Conjugation)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
25 µg/100 µg
| 规格: | 25 µg | 产品价格: | ¥1740.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥5590.0 |
EpCAM/TROP1重组蛋白|Recombinant Human EpCAM Protein (ECD, His Tag, Site-Specific AF488 Conjugation)(产品说明)
蛋白名称:Human EpCAM / TROP1 Protein (ECD, His Tag, Site-Specific AF 488 Conjugation)
蛋白构建:A DNA sequence encoding the Human EpCAM (NP_002345.1) (Met1-Lys265) was expressed with a polyhistidine tag at the C-terminus. The protein is site-specifically labeled with AF 488 (Excitation Max. = 495 nm, Emission Max. = 519 nm).
表达宿主:HEK293 Cells
蛋白纯度:≥ 90 % as determined by SDS-PAGE.
蛋白活性:1.Flow cytometric analysis of anti-EpCAM CAR expression. 293 cells were lentivirally transduced with anti-EpCAM CAR. Flow cytometric analysis was performed with a negative control protein and AF 488-conjugated human EpCAM protein (site-specific AF 488 conjugation, ECD, his tag, Cat. No. 10694-H86H-SD), respectively. Non-transduced 293 cells were used as a control. 2.Binding activity of PE-labeled EpCAM protein to PBMC cells. PBMC cells were stained with anti-CD3 antibody and AF 488-conjugated human EpCAM protein (ECD, his tag, site-specific AF488 conjugation, Cat. No. 10694-H86H-SD) and detected by flow cytometry. PBMC cells stained with anti-CD3 antibody were used as a control.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 24
蛋白分子量:The recombinant Human EpCAM consists of AA Met1-Lys265 with a polyhistidine tag at the C-terminus. It has a predicted molecular mass of 30.9 kDa.
蛋白NP号:NP_002345.1
蛋白氨基酸序列:Met1-Lys265
蛋白标签:C-His
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2与3、5与6之间有一个环状结构,是水通过的通道。另外,AQP1的氨基端和羧基端的氨基酸序列是严格对称的,因此,同源跨膜区(1,4、2,5、3,6)在质膜的脂双层中的方向相反。 AQP1对水的通透性受氯化汞的可逆性抑制,对汞的敏感位点是结构域5与6之间的189位的半胱氨酸。其他几种AQP1与肾功能有关。 49、运输ATPase(transport ATPase) 能够水解ATP,并利用ATP水解释放出的能量驱动物质跨膜运输的运输蛋白称为运输ATPase, 由于它们能够进行逆浓度梯度运输, 所以有称为泵。共有
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