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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CLDN4-VLP (Full Length) Protein (Flag Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg/100 µg/500 µg
| 规格: | 20 µg | 产品价格: | ¥6470.0 |
|---|---|---|---|
| 规格: | 100 µg | 产品价格: | ¥13620.0 |
| 规格: | 500 µg | 产品价格: | ¥47940.0 |
蛋白名称:Human CLDN4-VLP (Full Length) Protein (Flag Tag)
蛋白构建:A DNA sequence encoding the Human CLDN4-VLP (Full Length) (O14493) (Met1-Val209) was fused with a signal peptide at the N-terminus and a flag tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白活性:Immobilized Recombinant Human Claudin-4-VLP Protein (Cat:16358-H25H) at 5 μg/mL (100 μL/well) can bind Anti-Human Claudin-4 Monoclonal antibody, Human IgG1, the EC50 is 10-30 ng/mL.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met 1
蛋白分子量:The recombinant Human CLDN4-VLP (Full Length) consisting of 222 amino acids and has a calculated molecular mass of 23.39 kDa.
蛋白NP号:O14493
蛋白氨基酸序列:Met1-Val209
蛋白标签:C-Flag
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文献和实验在免疫沉淀IP&免疫荧光IF如何正确的选择Flag标签抗体? Flag标签系统是已被公认的用于表达、纯化以及检测融合蛋白的表达系统,广泛应用于Western blotting、免疫细胞组化、免疫共沉淀、流式细胞术、蛋白纯化以及蛋白相互作用、蛋白分离等多个研究领域。Flag标签是一个与重组蛋白相融合的由8个亲水氨基酸(Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)组成的多肽片断。FLAG标签只有8个氨基酸组成,因此它不会占据其他表位与结合域,避免导致融合蛋白
Anti-claudin-4, whose corresponding antigen receptors are known to be overexpressed in both primary and metastatic human pancreatic cancer, is utilized for targeted delivery and imaging of pancreatic cancer. In this protocol
Strep/FLAG Tandem Affinity Purification (SF‐TAP) to Study Protein Interactions
a methodological workflow based on an SF?TAP tag comprising a doublet Strep?tag II and a FLAG moiety optimized for rapid as well as efficient tandem affinity purification of native proteins and protein complexes in higher eukaryotic cells. Depending
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