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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
普洛麦格
- 英文名：
Red Blood Cell Lysis Solution (CLB)
- 规格：
200ml
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文献和实验Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis
) from erythrocytes and �granulocytes. However, this method is difficult to standardize especially under GMP conditions. Here, we describe an alternative approach for isolation of human MSC based on red blood cell (RBC) lysis with ammonium chloride. This results
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
until the pellet is white. Note: If some red blood cells or cell debris are still visible along with the white blood cell pellet, resuspend the white blood cell pellet and mix with 2 volumes ERL Buffer. Incubate 2 min at room temperature. Then pellet
BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method
by centrifugation at 5K rpm for 15 minutes in a 250 ml bottle in the RC5-B using the GSA rotor, cell pellets should be frozen and stored at -70 degrees C. 2、Prior to use, the cells from 200 ml growth were thawed and resuspended in 8 ml of just 10 mM of EDTA, pH
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