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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见产品外包装
- 英文名:
Recombinant Human Creatine Kinase MM/CKMM/CKM/creatine kinase, muscle
- 库存:
20
- 供应商:
上海吉至生化科技有限公司
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
microarrays per kinase to be probed (e.g., from protocol 2 ) Superblock/0.1% Triton X‐100 (Thermo/Fisher) Kinase to be assayed
Medium-Throughput Production of Recombinant Human Proteins: Protein Production in Insect Cells
and a screening process established in 2007 as a measure to tackle the more challenging kinase, RNA–DNA processing and integral membrane protein families on our target list. Here, we discuss our platform for identifying soluble proteins from 3 ml of insect cell
In vitro transcription with yeast nuclear extract
buffer 0.63 microliters 0.1 M DTT 3 microliters phospho creatine (64 mg/ml pH 7.5) 0.1 microliter creatine phospho kinase 1 microliter NTP mix (10 mM each ATP, CTP, GTP, UTP) 150 ng plasmid DNA template(0.125 microliters 10% NP40 can be added
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