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- 文献和实验
- 技术资料
- 保存条件：
-20°C. Desiccate
- 保质期：
2年
- 英文名：
AZDye 430 Picolyl Azide-25 mg
- 库存：
100
- 供应商：
艾美捷科技
- CAS号：
查看datasheet
AZDye 430Azide吡啶-25 mg，AZDye 430 Picolyl Azide-25 mg，AZDye 430Azide吡啶-25 mg,AZDye 430 Picolyl Azide-25 mg
AZDye 430Azide吡啶-25 mg-AZDye 430 Picolyl Azide-25 mg
产品货号：CCT-1272-25
产品规格：25mg
AZDye™ 430 Picolyl Azide是一种先进的荧光探针，它结合了铜螯合基序，以提高反应位点处Cu(I)的有效浓度，从而提高CuAAC反应的效率，从而实现更快、更生物相容的CuAAC标记。据报道，与常规叠氮化物相比，信号强度增加高达40倍。
AZDye™ 430 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported .In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.AZDye™ 430 is structurally identical to Alexa Fluor® 430. Its absorption/emission spectra is a perfect match to spectra of many other structurally similar dyes, including Alexa Fluor® 430 and CF®430 Dye.For application where the presence of copper is not acceptable, please consider our AZDye™ 430 DBCO probe for copper-less detection of azide-modified molecules.Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc
AZDye 430Azide吡啶-25 mg-AZDye 430 Picolyl Azide-25 mg-20°C. Desiccate
点击：AZDye 430Azide吡啶-25 mg-AZDye 430 Picolyl Azide-25 mg查看更详细产品说明，更多应用、储存、价格、货期等信息请垂询艾美捷科技有限公司。更多Vectorlabs公司Azides（Biotin-Azides）试剂、Bis-sulfone交联试剂、DBCO试剂、TCO试剂、Terminal Alkyne试剂、Tetrazine试剂、标记试剂盒以及分选用底物等。，请点击查看艾美捷特色产品中心。
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文献和实验Replication timing by density transfer
. (I add 0.1 mg/ml ampicillin if I'm feeling paranoid.) The doubling time of RM14-3a is ~ 2.6 hr at 23o C in glucose and 6-8 hr (depending on aeration) in acetate--longer if you're selecting for a plasmid. RM14-3a is MATa, cdc7-1ts , bar1, trp1-289
Replication timing by comparative hybridization
cells per time point. (Raffinose has not worked well for me, so I avoid it.) 2. Arrest the culture with alpha factor (200 nM final concentration for bar1 strains) when the OD660 is about 0.2-0.25. 3. When the cells have arrested (more than 95
Purification of Antiserum or Ascites by Protein A/G Chromatography
1、Required Materials and Equipment(1) Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A or protein G)(2) Ice-cold Tris-buffered saline (TBS). See recipe below(3) 5% sodium azide solution
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