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文献和实验Analysis of Proteins using Small Format 2D Gel Electrophoresis
twice with PBS-A and lysed in 0.2 ml of 2D lysis buffer (0.01M Tris-HCl, pH 7.4, 1mM EDTA, 8M urea, 0.05M dithiothreitol, 10% (v/v) glycerol, 5% (v/v) Nonidet P-40, 200µg/ml RNAse A, 6% (w/v) pH 3.5-10 carrier ampholytes ("Resolyte", Merck
. and Becher, G. 2000. Synthesis, base pairing, and fluorescence properties of oligonucleotides containing 1H‐pyrazolo[3,4‐d]pyrimidin‐6‐amine (8‐aza‐7‐deazapurin‐2‐amine) as an analogue of purin‐2‐amine. Helv. Chim. Acta 83:928‐942
Analysis of Proteins using Small Format 2D Gel Electrophoresis
-A and lysed in 0.2 ml of 2D lysis buffer (0.01M Tris-HCl, pH 7.4, 1mM EDTA, 8M urea, 0.05M dithiothreitol, 10% (v/v) glycerol, 5% (v/v) Nonidet P-40, 200µg/ml RNAse A, 6% (w/v) pH 3.5-10 carrier ampholytes ("Resolyte", Merck Ltd.)) for 5 minutes on ice
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