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- 2025年09月25日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
Guava ViaCount Reagent (40 mL)
- CAS号：
4000-0040
- 保质期：
2年
- 供应商：
上海瓦兰生物
- 保存条件：
2-8℃
- 规格：
40ml
Description
The Guava® ViaCount™ reagent allows for the quantitative analysis of cell count and viability. The reagent provides precise, accurate assessments on a wide variety of cell lines, even those with unusual culture conditions or a tendency to aggregate.
The ViaCount reagent distinguishes viable and non-viable cells based on differential permeabilities of two DNA-binding dyes in the Guava ViaCount reagent. The nuclear dye stains only nucleated cells, while the viability dye brightly stains dying cells. This proprietary combination of the dyes enables Guava ViaCount to distinguish viable, apoptotic, and dead cells. Debris is excluded from results based on negative staining with the nuclear dye.
Using as little as 20 µL of sample, the ViaCount reagent provides the following:
- Total absolute cell counts
- Viability counts and percentages
- Apoptotic percentages and counts
The assay has proven to be more accurate than hemocytometer counting and impedence-based counting over a wide range of cell concentrations without the use of expensive reference beads. By providing data on viable, dead, and apoptotic cell populations in a single measurement, the ViaCount reagent enables a more precise evaluation of culture conditions and cell viability to better optimize culture conditions.
Precise, accurate cell counts provide consistent results for experiments that depend upon accurate determination of viable cells for success such as ELISpot, cell-based fluorescence assays, and cytokine production assays.
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文献和实验用于Northern杂交、斑点杂交、mRNA纯化、体外翻译、RNA分解酶的保护分析、RT-PCR、构建cDNA文库等各种分子生物学实验。【试剂盒之外所需准备试剂】氯仿、异丙醇、75% 乙醇（ D E P C 处理水配制) 、RNase-free水（制备方法：使用RNase-free的玻璃瓶，向超纯水中加DEPC至终浓度0.01%（v/v)，过夜搅拌后，高温高压灭菌。）保存和运输1. RNAiso Reagent在室温下可以保存12个月，为保证试剂质量，建议于2-8℃下避光保存。2. 可以在常温下运输。RNA
isolated from the other components. PCR products should be analyzed in an area separate from the reaction assembly area. 1) Add the following components to a sterile 0.5-mL microcentrifuge tube on ice: 2) Cap the tubes and centrifuge briefly
Western blotting using ECL reagent
diluted 1:8000 in TBS-T + 1.5% gelatin for 1 hour.6. Repeat step # 4 (wash)7. Prepare ECL solution for detection: mix equal volume of ECL reagent 1 and 2, (1:1) with final volume regarding of 0.125 ml/cm2 (for a mini gel- 4 ml of each reagent).8. Remove
